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Emission lumineuse du système Fe2 + -EDTA-acide ascorbique-H2 O2 fortement renforcée par les acides phénoliques végétaux.

Les réactions d’oxydation peuvent entraîner la formation d’espèces excitées électroniquement qui subissent une désintégration radiative en fonction de la transition électronique de l’état excité à l’état fondamental, suivie d’une émission de photons ultra-faible (UPE). Nous avons étudié l'UPE du système Fe (2+) -EDTA (acide éthylènediaminetétraacétique) -AA (acide ascorbique) -H2O2 (peroxyde d'hydrogène) avec un luminomètre à tubes multiples (compteur de photons refroidi par Peltier, gamme spectrale de 380 à 630 nm). L’UPE, de 92,6 mol / L Fe (2+), 185,2 mol / EDTA, 472 mol / L AA, 2,6 mmol / L H2 O2, a atteint 1217 ± 118 unités de lumière relative pendant une mesure de 2 min et était d'environ deux fois plus élevé (P <0,001) que l'UPE des systèmes incomplets (Fe (2+) -AA-H2 O2, Fe (2+) -EDTA-H2 O2, AA-H2 O2) et du milieu seul. Substitution de Fe (2+) avec du Cr (2+), du Co (2+), du Mn (2+) ou du Cu (2+) ainsi que de l'EDTA avec de l'EGTA (éthylène glycol-bis (beta-aminoéthyl éther)) - N, N, N ', N'-tétraacétique) ou un citrate puissamment inhibé UPE. Des expériences avec des agents de piégeage d'espèces réactives de l'oxygène (diméthylsulfoxyde, mannitol, azoture de sodium, superoxyde dismutase) ont révélé la dépendance de l'EPU uniquement vis-à-vis des radicaux hydroxyles. Le diméthylsulfoxyde à la concentration de 0,74 mmol / L a inhibé l'UPE de 79 ± 4%. Les composés phénoliques végétaux (acides férulique, chlorogénique et caffec) à la concentration de 870 mmol / L ont fortement augmenté l'EPU de 5, 13,9 et 46,8 fois (P <0,001), respectivement. Il est suggéré que l’augmentation de l’UPE provenant du système Fe (2+) -EDTA-AA-H2 O2 puisse être appliquée à la détection de ces substances phytochimiques.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30675979


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