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Mélange de nanoparticules d'or / MnO2 @ BSA pour la détermination par résonance fluorométrique et magnétique de l'acide ascorbique.

Un schéma de détection bimodale à résonance fluorométrique et magnétique (MR) est présenté pour la détermination de l'acide ascorbique (AA). Il est basé sur l'utilisation d'un mélange mélangé Au / MnO2 @ BSA préparé selon une stratégie biomimétique, en utilisant de la sérumalbumine bovine (BSA) comme matrice à la température physiologique. La fraction MnO2 @ BSA (une partie du composite) n'est pas sensible à la MR, mais peut être dégradée en composés actifs de la MR lors d'une réaction redox avec des concentrations même ultra-faibles en AA. En parallèle, le mélange Au / MnO2 @ BSA récupère sa fluorescence car MnO2 @ BSA agit comme un extincteur de la fluorescence de Au @ BSA circonjacent (l'autre partie du composite). La fluorescence est généralement mesurée aux longueurs d'onde d'excitation / émission de 470/625 nm. En exploitant cette réaction d'oxydo-réduction entre MnO2 et AA, un schéma de détection à double mode pour l'AA a été développé. La fluorescence et le signal MR augmentent tous les deux avec la concentration en AA. La limite la plus basse pour la détection de AA est de 0,6 µM en mode fluorométrique et de 0,4 µM en mode MR. L'analyse d'échantillons de sérum enrichis en AA a montré que les récupérations obtenues en mode fluorométrique et en mode MR peuvent atteindre 94%. Ceci est le premier rapport sur l'utilisation de nanoparticules mélangées avec leur régularité inhérente de validation croisée. Résumé graphique Présentation schématique de la synthèse biomimétique de nanosondes Au / MnO2 @ BSA mélangées et de la détection bimodale fluorométrique / MR à validation croisée de l'acide ascorbique.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30631952


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