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L'acide ascorbique diminue la différenciation ostéogénique induite par la BMP-2 des cellules précurseurs musculaires.

INTRODUCTION: Les cellules précurseurs musculaires (MPC) font partie intégrante du maintien du muscle squelettique et ont récemment été impliquées dans la réparation osseuse. L'objectif principal de cette étude était de mieux comprendre le rôle du stress oxydatif au cours de la différenciation ostéogénique des MPC. Méthodes: Les MPC ont été traitées avec diverses combinaisons d'acide ascorbique (AA), de BMP-2 et d'un analogue de la superoxyde dismutase (TEMPOL) ou de la catalase conjuguée à du polyéthylèneglycol. La prolifération et la différenciation des MPC ont été déterminées et l'activité de la phosphatase alcaline a été mesurée en tant qu'indice de différenciation ostéogénique. RÉSULTATS: 200 µm AA ont augmenté le superoxyde 1,5 fois plus, tandis que l'AA associé à 100 ng / ml de BMP-2 n'a pas augmenté l'activité de la phosphatase alcaline (ALP). Lorsque les cellules ont été traitées avec TEMPOL en association avec 100 ng / ml de BMP-2 et 200 μmA, l'activité de la PAL augmentait de manière significative. DISCUSSION: Ensemble, ces données suggèrent que l'augmentation du stress oxydatif associé à l'AA induit un stress oxydatif sublétal qui empêche la différenciation ostéogénique induite par la BMP-2 des MPC. Cet article est protégé par le droit d'auteur. Tous les droits sont réservés.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30623463


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