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L'acide ascorbique améliore la fonction thrombotique des plaquettes lors d'une greffe du foie d'un donneur vivant en modulant la fonction des ubiquitine ligases E3 c-Cbl et Cbl-b.

OBJECTIF: Etudier l'effet de l'acide ascorbique (AA) sur la fonction hémostatique lors d'une transplantation de foie d'un donneur vivant (LDLT). MÉTHODES: Des échantillons de sang prélevés sur 21 receveurs de LDLT ont été prélevés dans les 30 minutes suivant l'induction et dans les 120 minutes suivant la reperfusion. Une thromboélastographie par rotation (TEG) et une analyse par transfert Western ont été utilisées pour analyser la fibrinolyse et les modifications fonctionnelles des c-Cbl et Cbl-b, respectivement. Les échantillons de test TEG ont été préparés en trois groupes: groupe C (0,36 ml de sang), groupe N (0,324 ml de sang + 0,036 ml de solution saline normale à 0,9%) et groupe A (0,324 ml de sang + 0,036 ml de 200 micromol / L-AA dissous dans une solution saline normale à 0,9%). RÉSULTATS: Les AA ont diminué la fibrinolyse et augmenté la rigidité du caillot au début et 120 minutes après la reperfusion. L'expression de Cbl-b était significativement augmentée au début et 120 minutes après la reperfusion dans le groupe A par rapport aux groupes C et N. Cependant, la phosphorylation de c-Cbl était la plus significativement diminuée dans le groupe A au début et 120 minutes après la reperfusion. CONCLUSION: Les AA peuvent considérablement réduire la fibrinolyse et améliorer la rigidité du caillot chez les receveurs de LT au cours du LDLT, et des modifications fonctionnelles des Cbl-b et c-Cbl pourraient représenter le mécanisme sous-jacent. Un traitement par AA peut être envisagé pendant le LDLT pour diminuer l'hyperfibrinolyse.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30614340


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