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Identification et analyse des homologues de VTC2 codant pour l'enzyme clé de la biosynthèse de l'acide L-ascorbique chez des espèces de tomates (section Solanum de Lycopersicon).

Les séquences nucléotidiques complètes de 11 homologues de GDP-L-galactose-phosphorylase-1 de tomates sauvages et cultivées (VTC2) ont été identifiées et caractérisées. Le niveau global de variabilité des nucléotides était de 9,19%. Au total, 25 SNP de l'ADNc sur 54 n'étaient pas synonymes et ont entraîné des substitutions d'acides aminés. Dans le groupe des espèces de tomates à fruits verts, plus de SNP ont été détectés que dans le groupe des espèces à fruits rouges, mais le pourcentage de substitutions non synonymes dans les tomates à fruits rouges était significativement plus élevé (66,7% contre 37,5%). Dans les séquences d'acides aminés traduites, le domaine GDF-L-galactose-hexose-1-phosphate-guanyltransférase et le motif HIT hautement conservé ont été identifiés et un motif conservé spécifique de VTC2 des espèces de Solanoideae a été détecté.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30607742


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