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Filename: drivers/Session_files_driver.php
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Filename: drivers/Session_files_driver.php
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Filename: drivers/Session_files_driver.php
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Filename: Session/Session.php
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Function: __construct
File: /home/orthodiedw/nutridatabase/index.php
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La détection de l'acide ascorbique (AA) dans les échantillons biologiques et dans les échantillons d'aliments est essentielle pour la santé humaine. Ici, une nanosonde fluorescente ratiométrique ratiométrique à base de MnO2 (MnO2-NS) a été développée pour la détection hautement sensible et spécifique des AA. Le MnO2-NS présente une activité semblable à celle de la peroxydase et peut oxyder le substrat non fluorescent de l'o-phénylènediamine (OPDA) en substrat fluorescent, présentant une fluorescence maximale à 568 nm (F568). Si MnO2-NS est prémélangé avec AA, le MnO2-NS est alors décomposé en Mn (2+) par AA, ce qui diminue l'intensité de fluorescence de F568. En attendant, l'AA est oxydé sous forme d'acide déhydroascorbique (DHAA), qui peut réagir avec l'OPDA pour générer un substrat fluorescent. Une nouvelle réponse de fluorescence est trouvée à 425 nm (F425). Les réponses doubles fluorescentes peuvent être excitées avec une longueur d'onde d'excitation universelle, simplifiant la procédure de détection. Avec F425 / F568 en lecture, la limite de détection pour AA atteint aussi peu que 10,0 nM. Des taux de récupération satisfaisants sont trouvés pour la détection des AA dans le sérum et diverses boissons. La stratégie ratiométrique élimine de manière significative les résultats faussement négatifs et faussement positifs, offrant ainsi un moyen rentable, rapide et fiable de détecter l'AA dans un échantillon réel.
Source: https://www.ncbi.nlm.nih.gov/pubmed/30406417