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Synthèse et optimisation de nanoparticules de chitosan chargées en acide L-ascorbique et thymoquinone.

La combinaison de composés de différentes classes (caractères hydrophobes et hydrophiles) dans un seul support de chitosane est un défi en raison du caractère hydrophile du chitosane. L'utilisation des composés de l'acide l-ascorbique (LAA) et de la thymoquinone (TQ) en tant qu'antioxydants efficaces est gâchée par une faible biodisponibilité et absorption. Les nanoparticules (NP) ont résolu le problème en fonctionnant comme un support car elles ont une grande surface pour une libération et une absorption plus efficaces par les cellules. Cette recherche a donc synthétisé des NP de chitosane (CNP) contenant LAA et TQ, CNP-LAA-TQ via des voies de gélification ionique car la préparation est non toxique. Ils ont été caractérisés par microscopie électronique, zetasizer, spectrophotométrie UV (-) VIS et spectroscopie infrarouge. La taille optimale de CNP-LAA-TQ produite était de 141,5 ± 7,8 nm, avec un indice de polydispersité (PDI) de 0,207 ± 0,013. L’efficacité d’encapsulation de CNP-LAA-TQ était de 22,8 ± 3,2% pour la LAA et de 35,6 ± 3,6% pour le TQ. La LAA hydrophile combinée et le TQ hydrophobe ont prouvé qu'une myriade de composés hautement efficaces avec une absorption systémique médiocre pouvaient être encapsulés ensemble dans des systèmes NP afin d'accroître leur efficacité pharmaceutique, contribuant ainsi indirectement au progrès des secteurs médical et pharmaceutique.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30405074


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