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L'effet protecteur exercé par l'acide ascorbique sur la fragmentation de l'ADN de leucocytes humains induite par le venin de Lachesis muta muta.

L’objectif de cette étude était d’évaluer les effets génotoxiques et mutagènes des toxines présentes dans le venin de Lachesis muta muta sur les leucocytes du sang périphérique humain et le potentiel de protection de l’acide ascorbique sur la fragmentation de l’ADN. Le venin de L. muta muta a été incubé à différentes concentrations (1, 2,5, 5, 7,5, 10, 15, 20, 30, 40, 50, 60 et 120 microg / mL) avec du sang humain pour évaluer la fragmentation de l'ADN à l'aide du électrophorèse sur comète, gel d’agarose et micronoyau. À ces concentrations évaluées, le venin de la mutabilité induite par L. muta muta (test des comètes et électrophorèse sur gel d'agarose) et la mutagénicité (test du micronoyau), mais ils n'étaient pas cytotoxiques, car ils ne modifiaient pas le taux de prolifération cellulaire après blocage de la cytokinèse par la cytochalasine B. L'acide ascorbique inhibe de manière significative la génotoxicité induite par le venin de L. muta muta dans les proportions évaluées (1: 0,1 et 1: 0,5, venin / acide ascorbique - poids / poids). Ainsi, de futures études sont nécessaires pour élucider les mécanismes de protection de l'acide ascorbique sur les effets génotoxiques induits par les toxines présentes dans les venins de serpents.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30321470


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