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Une stratégie fluorescente simple et pratique pour la détection hautement sensible de la dopamine et de l’acide ascorbique à base de points quantiques de graphène.

Dans cet article, une stratégie d’activation fluorescente simple et pratique pour la détection très sensible de la dopamine (DA) et de l’acide ascorbique (AA) sur la base de points quantiques en graphène (GQD) a été développée. Les DA et les GQD peuvent former le complexe DA-GQD en raison de l'interaction électrostatique et de la liaison hydrogène. L'interaction entre Cu (2+) et le fragment catéchol du complexe DA-GQD pourrait conduire à une forte atténuation de la fluorescence des GQD. Lorsque l'AA est introduit dans le système, l'AA peut réduire le Cu (2+) en Cu (+), perturbant ainsi l'interaction entre le complexe Cu (2+) et le complexe DA-GQD et entraînant la récupération de la fluorescence des GQD. Ainsi, un capteur sensible et pratique pour la détection de DA et AA a été développé. Dans les conditions expérimentales optimisées, l’intensité de la fluorescence a été corrélée linéairement avec les concentrations de DA et AA comprises entre 0,5 et 120 micromolL (-1) et 0,05 à 6 micromolL (-1) avec une limite de détection de 0,16 micromolL (-1) et 0,021 micromolL (-1), respectivement. En attendant, la méthode proposée a des applications potentielles sur la détection de DA et AA dans des échantillons d'urine et de sérum humains.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30086905


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