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Utilisation de l'analyse par injection séquentielle avec laboratoire à la valve et sonde optique pour la détermination spectrophotométrique simultanée de l'acide ascorbique et de la cystéine par centrage moyen des profils cinétiques de rapport.

Deux nouvelles méthodes simples, relativement rapides et robustes de détermination cinétique simultanée de mélanges binaires d'acide ascorbique (Asc) et de cystéine (Cys) ont été mises au point à l'aide de la méthode du centrage moyen des profils cinétiques. Les méthodes sont basées sur la différence entre les vitesses de réaction de Asc et Cys avec le 18-molybdodiphosphate à pH 5,1. Une sonde optique ainsi que la méthode SIA-LAV (laboratoire d’injection par injection séquentielle) ont été utilisées pour effectuer une analyse cinétique simultanée. Les avantages de la méthode du centrage moyen des profils cinétiques par rapport ont été montrés par rapport aux autres méthodes cinétiques spectrophotométriques. Asc et Cys peuvent être déterminés dans les plages de concentrations comprises entre 20 et 200 et 8 à 90 micromolL (-1) avec la méthode spectrophotométrique en discontinu et 10-200 et 4-40 micromolL (-1) avec la méthode SI-LAV, respectivement. La méthode a été utilisée avec succès pour déterminer Asc et Cys dans des compléments alimentaires.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30029453


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