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File: /home/orthodiedw/nutridatabase/application/controllers/Articles.php
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File: /home/orthodiedw/nutridatabase/application/controllers/Articles.php
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File: /home/orthodiedw/nutridatabase/application/controllers/Articles.php
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File: /home/orthodiedw/nutridatabase/application/controllers/Articles.php
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File: /home/orthodiedw/nutridatabase/application/controllers/Articles.php
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Un nouveau facteur de transcription MYB régule la synthèse de l'acide ascorbique et affecte la tolérance au froid.

La déshydroascorbate réductase (DHAR) joue un rôle important dans les réponses au stress, mais la régulation transcriptionnelle de DHAR en réponse au stress abiotique est encore mal comprise. Dans cette étude, nous avons isolé un nouveau facteur de transcription MYB de type R2R3 à partir de Pyrus betulaefolia par criblage à un hybride dans une levure, désigné par PbrMYB5. PbrMYB5 était localisé dans le noyau et pouvait se lier spécifiquement au promoteur de PbrDHAR2. PbrMYB5 était fortement induit par le froid et le sel, mais légèrement par la déshydratation. La surexpression de PbrMYB5 dans le tabac a conféré une tolérance accrue aux stress dus au froid, tandis que la régulation négative de PbrMYB5 chez P. betulaefolia par silençage du gène induit par le virus a entraîné une sensibilité accrue au froid. Le tabac transgénique présentait des niveaux d'expression de NtDHAR2 plus élevés et accumulait une plus grande quantité d'acide ascorbique (AsA) que les plantes de type sauvage. Le silençage du gène induit par le virus de PbrMYB5 dans l'abondance de PbrDHAR2 régulée à la baisse par P. betulaefolia et une diminution du niveau d'AsA, accompagnés d'une sensibilité accrue au stress de refroidissement. Pris ensemble, ces résultats démontrent que PbrMYB5 est un activateur de la biosynthèse de l'AsA et peut jouer un rôle positif dans la tolérance au froid, du moins en partie, en raison de la modulation de la synthèse de l'AsA en régulant l'expression de PbrDHAR2.


Source: https://www.ncbi.nlm.nih.gov/pubmed/29929211


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