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[Méthode basée sur la chimiluminescence électrogénérée de Ru (bpy) 3 3+ pour l'évaluation de la propriété antioxydante d'antioxydants naturels].

Nous avons développé une méthode d'analyse par injection de flux (FIA) basée sur la chimiluminescence électrogénérée de tris (2,2'-bipyridine) ruthénium (III) [Ru (bpy) 3 (3+)] pour l'évaluation de la propriété antioxydante. Le radical hydroxyle (OH) a été généré par photolyse de H2O en utilisant un photoréacteur à ultraviolets / H2O. Le réacteur comprenait un tube en polytétrafluoroéthylène, un récipient en quartz et une lampe à mercure à basse pression émettant principalement des radiations à environ 185 nm. Lorsque le radical hydroxyle et Ru (bpy) 3 (3+) étaient en contact, une chimioluminescence a été générée en tant qu'émission de fond. L'émission de fond a diminué lorsque des échantillons d'antioxydant ont été injectés dans le système. Les propriétés antioxydantes des antioxydants naturels testés sont énumérées ici, en commençant par le plus élevé: acide gallique> acide ascorbique> quercétine. De plus, notre méthode permettait un débit d'environ 100 échantillons / h. La méthode proposée à haut débit peut être utilisée pour évaluer la propriété antioxydante des antioxydants naturels.


Source: https://www.ncbi.nlm.nih.gov/pubmed/30713243


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